Primer Match

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Primer Match is a program that identifies primer binding locations on the forward and reverse strand of a target sequence.

Primers are given in FASTA format. More than one primer may be given.

Target sequence is given in FASTA format. One sequence should be given. The program will automatically calculate the reverse complement.

Primer match length is the minimum length on the 3' region of the primer that needs an exact match.

Permitted 5' overhanging length is the minimum length of overhanging region of the primer.

Primers (FASTA Format) Target Sequence (FASTA Format) Primer match length Permitted 5' overhanging length 




Acknowledgements: Dr Josh Mylne for suggesting the idea