vigno 5(786) Protein Card

General Information
Name vigno 5
Class Cyclotide
Technique MS
Average Mass 2860.28
Monoisotopic Mass 2858.15
m/z M+H 2860.28
ProteinType Wild type
Organism Viola tricolor
Viola ignobilis
Notes Induces cell death in HeLa line at micromolar concentrations (Esmaeili et al., 2016). [Control data not reported]. Apoptotic markers (chromatin condensation, DNA fragmentation, phosphatidyl serine exposure, loss of mitochondrial membrane potential). Mitochondrial (intrinsic) apoptotic pathway implicated. Fibroblast cell line was less susceptible to cell death when treated with this peptide. Can be extracted from plant material by microwave-assisted extraction, less efficient than maceration. (Farhadpour et al., 2016). Linear form identified in Viola tricolor extract. (Hellinger et al., 2015)
Cyclic Yes

No assays found

Hashempour,H., Koehbach,J., Daly,N.L., Ghassempour,A. and Gruber,C.W. (2013) Characterizing circular peptides in mixtures: sequence fragment assembly of cyclotides from a violet plant by MALDI-TOF/TOF mass spectrometry. Amino Acids 44:581-595
Hellinger,R., Koehbach,J., Soltis,D.E., Carpenter,E.J., Wong,G.K. and Gruber,C.W. (2015) Peptidomics of Circular Cysteine-Rich Plant Peptides: Analysis of the Diversity of Cyclotides from Viola tricolor by Transcriptome and Proteome Mining. J. Proteome Res. 14:4851-4862
Esmaeili,M.A., Abagheri-Mahabadi,N., Hashempour,H., Farhadpour,M., Gruber,C.W. and Ghassempour,A. (2016) Viola plant cyclotide vigno 5 induces mitochondria-mediated apoptosis via cytochrome C release and caspases activation in cervical cancer cells. Fitoterapia 109:162-168
Farhadpour,M., Hashempour,H., Talebpour,Z., A-Bagheri,N., Shushtarian,M.S., Gruber,C.W. and Ghassempour,A. (2016) Microwave-assisted extraction of cyclotides from Viola ignobilis. Anal. Biochem. 497:83-89

Nucleic acids