[T16A]kalata B1(466) Protein Card

General Information
Name [T16A]kalata B1
Sequence GLPVCGETCVGGTCNAPGCTCSWPVCTRN
Class Cyclotide
Average Mass 2862.25
Monoisotopic Mass 2860.13
m/z M+H 2861.14
ProteinType Mutant
Parent kalata B1
Organism Synthetic
Notes
Cyclic Yes

Assay
Insecticidal
Substitution of certain residues of kalata B1 to alanine result in loss of insecticidal activity [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Hemolytic
Substitution of certain residues of kalata B1 to alanine result in loss of hemolytic activity. Residues in loops 1 and 3 along with a few other isolated residues strongly affect activity. [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Metal binding assay
In the presence of Mn2+ kalata B1, T16A and T27A showed specific broadening of a defined set of amide signals. There was almost complete broadening of the amide signals from C14, N15, and T16/A16, and significant broadening (i.e. ~70% attenuation) of loop 1 residues. By contrast, E7A showed significantly less broadening. [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Nematocidal
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589

References
Simonsen SM, Sando L, Rosengren KJ, Wang CK, Colgrave ML, Daly NL, Craik DJ (2008) Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity J Biol Chem 283:9805-9813

Cross-references
Nucleic acids
Structure
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