MCoTI-II[K10F](343) Protein Card

General Information
Name MCoTI-II[K10F]
Sequence SGSDGGVCPFILKKCRRDSDCPGACICRGNGYCG
Class Squash trypsin inhibitor
Average Mass 3471.94
Monoisotopic Mass 3469.47
m/z M+H 3471.94
ProteinType Mutant
Parent MCoTI-II
Organism Synthetic
Notes
Cyclic Yes

Assay
Protease inhibition
Synthetic MCoTI-I and MCoTI-II are both extremely potent trypsin inhibitors. [K10R] mutant has essentially wild-type activity against trypsin, whilst [K10F] and [K10Q] mutants are less active. [K10F] is the only mutant to show significant activity against chymotrypsin in this assay. [...]Thongyoo P et al. (2007) Chembiochem 8:1107-1109

References
Thongyoo P, Jaulent AM, Tate EW, Leatherbarrow, RJ (2007) Immobilized protease-assisted sysnthesis of engineered cysteine-knot microproteins Chembiochem 8:1107-1109

Cross-references
Nucleic acids
Structure
Links

Tools