kalata B7(26) Protein Card

General Information
Name kalata B7
Class Cyclotide
Technique Amino acid analysis,EST,MS,NGS
Average Mass 3071.54
Monoisotopic Mass 3069.27
m/z M+H 3071.54
ProteinType Wild type
Organism Oldenlandia affinis
Geophila repens
Cyclic Yes

Membrane Binding Assay
Kalata B7 binds to DPC micelles [...]Shenkarev ZO et al. (2008) J Inorg Biochem 102:1246-1256
Metal binding assay
Kalata B7 binds to Mn2+ in the presence of DPC micelles [...]Shenkarev ZO et al. (2008) J Inorg Biochem 102:1246-1256
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
Cyclotides show molluscicidal activity [...]Plan MR et al. (2008) J Agric Food Chem 56:5237-5241

Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ (2004) Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. J Biol Chem 279:46858-67
Jennings C, West J, Waine C, Craik D, Anderson M (2001) Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis. Proc Natl Acad Sci U S A 98:10614-9
Plan MR, Goransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ (2007) The Cyclotide Fingerprint in Oldenlandia affinis: Elucidation of Chemically Modified, Linear and Novel Macrocyclic Peptides Chembiochem 8:1001-1011
Qin Q, McCallum EJ, Kaas Q, Suda J, Saska I, Craik DJ, Mylne JS. (2010) Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11:111-0
Keov,P., Liutkevi?i?t?,Z., Hellinger,R. and Gruber,C.W. (2018) Discovery of peptide probes to modulate oxytocin-type receptors of insects. Sci Rep 8:10020-0
Strömstedt,A.A., Kristiansen,P.E., Gunasekera,S., Grob,N., Skjeldal,L. and Göransson,U. (2016) Selective membrane disruption by the cyclotide kalata B7: complex ions and essential functional groups in the phosphatidylethanolamine binding pocket. Biochim Biophys Acta 1858:1317-1327
Rajendran,S., Slazak,B., Mohotti,S., Muhammad,T., Strömstedt,A.A., Kapusta,M., Wilmowicz,E., Göransson,U., Hettiarachchi,C.M., and Gunasekera,S. (2023) Screening for Cyclotides in Sri Lankan Medicinal Plants: Discovery, Characterization, and Bioactivity Screening of Cyclotides from Geophila repens. J Nat Prod 86:52-65

Protein precursor(s) kalata B7 precursor
Nucleic acids kalata B7 precursor [OaK3]
Structure NMR spatial srtucture of ternary complex kalata B7/Mn2+/DPC micelle
Solution structure of kalata B7
Links GenBank AAL05478.1
SwissProt P58457