[Ala1,15]kB1(23) Protein Card

General Information
Name [Ala1,15]kB1
Class Cyclotide
Average Mass 2830.18
Monoisotopic Mass 2828.21
m/z M+H 2829.22
ProteinType Mutant
Parent kalata B1
Organism Synthetic
Notes Kalata B1 with Cys 1 and Cys 15 mutated to Ala
Cyclic Yes

NOE Restraint
NOE restraints for 1N1U [...]
Chemical stability
Kalata B1 and B2 and acyclic permutants were found to be resistant to chemical denaturation in the oxidised state but not when reduced. [...]Colgrave ML and Craik DJ (2004) Biochemistry 43:5965-75
Chemical Shift
Chemical shift data for [Ala1,15]kB1 [...]Daly NL et al. (2003) J Biol Chem 278:6314-22

Colgrave ML, Craik DJ (2004) Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: the importance of the cyclic cystine knot. Biochemistry 43:5965-75
Daly NL, Clark RJ, Craik DJ (2003) Disulfide folding pathways of cystine knot proteins. Tying the knot within the circular backbone of the cyclotides. J Biol Chem 278:6314-22

Nucleic acids
Structure NMR structure of [Ala1,15]kalata B1