des(24-28)kB1(22) Protein Card

General Information
Name des(24-28)kB1
Sequence VCGETCVGGTCNTPGCTCSWPVCT
Class Cyclotide
Average Mass 2372.68
Monoisotopic Mass 2370.86
m/z M+H 2371.87
ProteinType Acyclic permutant
Parent kalata B1
Organism Synthetic
Notes Truncated acyclic permutant in loop 6
Cyclic No

Assay
Enzymatic Digest
Resistance to enzymatic digest shown for kalata B1 and acyclic permutants. Reduced protein was susceptible. [...]Colgrave ML and Craik DJ (2004) Biochemistry 43:5965-75
Resistance to enzymatic digest shown for kalata B1 and acyclic permutants. Reduced protein was susceptible. [...]Colgrave ML and Craik DJ (2004) Biochemistry 43:5965-75
Chemical stability
Kalata B1 and B2 and acyclic permutants were found to be resistant to chemical denaturation in the oxidised state but not when reduced. [...]Colgrave ML and Craik DJ (2004) Biochemistry 43:5965-75
Hemolytic
Resistance to enzymatic digest shown for kalata B1 and acyclic permutants. Reduced protein was susceptible. [...]Barry DG et al. (2003) Biochemistry 42:6688-95

References
Colgrave ML, Craik DJ (2004) Thermal, chemical, and enzymatic stability of the cyclotide kalata B1: the importance of the cyclic cystine knot. Biochemistry 43:5965-75
Barry DG, Daly NL, Clark RJ, Sando L, Craik DJ (2003) Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity. Biochemistry 42:6688-95

Cross-references
Nucleic acids
Structure Solution Structure of acyclic permutatnt des-(24-28)-kalata B1
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