(74) Assay Card

General Information
Summary Inhibited [125I] Neurotensin binding to HT-29 cell membranes (IC50 3 uM), and increased levels of cytosolic Ca2+ in two unrelated cell lines that do not express NT receptors
Condition Incubate HT-29 carcinoma cell membranes with 6 pM [125I]NT, 30 min, 1 mL. Terminated by vacuum filtration, quantified using gamma counter. Ca2+ monitored using Delta-scan Spectrofluometer.
Result IC50 3uM. Dose-dependently increase intracellular Ca2+ concentrations, which was not blocked by known NT antagonist. Increased Ca2+ in two unrelated cell lines that do not express NT receptors, therefore precludes as NT antagonist.
AssayType Neurotensin Inhibition

References
Witherup KM, Bogusky MJ, Anderson PS, Ramjit H, Ransom RW, Wood T, Sardana M (1994) Cyclopsychotride A, a biologically active, 31-residue cyclic peptide isolated from Psychotria longipes. J Nat Prod 57:1619-25

Cross-references
Proteins Assayed cyclopsychotride A