(70) Assay Card

General Information
Summary Disulfide bond is essenial for structure and function of acyclic SFTI-1. Seleno aSFTI-1 has 30% reduced inhibitory constant.
Condition At 25 degrees Celsius, 5 min incubation in 20 mM Tris, pH 7.8, 10 mM CaCl2. TAME substrate, absorbance at 247 nm.
Result Ki (M): Seleno aSFTI-1 (6.5E-9), aSFTI-1 (1.96E-9), [Ser3,11]aSFTI-1 (8.1E-6)
AssayType Protease inhibition

Guo X, Shi J, Tang Z, Cui D, Zhang Y (2006) Synthesis and biological activity of seleno sunflower trypsin inhibitor analog. Chem Biol Drug Des 68:341-4

Proteins Assayed Seleno aSFTI-1