(68) Assay Card

General Information
Summary SFTI-1 and analogues shown to inhibit Bovine beta-trypsin and Human leukocyte elastase
Condition Spectrophotometric titration with NPGB, using a modified Green-Work method. At 0.1 M Tris-HCL, pH 8.3, 20 mM CaCl2, 0.005% Triton X-100 at 22 degrees Celsius.
Result Acyclic variants Ka (M-1) for Bovine beta-trypsin and Human leukocyte elastase: SFTI-1 (9.9E10, nd), [Val5]SFTI-1 (na, 1.4E7), [Ser5]SFTI-1 (6.0E7, 1.3E6), [(R,S)HmVal5]SFTI-1 (7.0E8, 2.2E6), [(S)HmVal5]SFTI-1 (5.5E8, 0.7E6), [(R)HmVal5]SFTI-1 (6.5E8, 1.8E6), [HmSer5]SFTI-1 (1.4E9, 0.4E6)
AssayType Protease inhibition

Zablotna E, Kret A, Jaskiewicz A, Olma A, Leplawy MT, Rolka K (2006) Introduction of alpha-hydroxymethyamino acid residues in substrate specificity P1 position of trypsin inhibitor SFTI-1 from sunflower seeds retains its activity. Biochem Biophys Res Commun 340:823-8

Proteins Assayed [Val5]SFTI-1