(42) Assay Card

General Information
Summary Head-to-Tail cyclization improved biological activity of Src homology domain of c-Crk.
Condition The ligand-binding properties of the linear14 and cyclic Crk SH3 domains were studied by using a fluorescence-based binding assay.
Result Cyclization did not have a detrimental effect on ligand-binding activity; on the contrary, the circular SH3 domain had a ~7-fold higher affinity for the ligand than the linear SH3 domain (Kd[circular] = 0.43 ± 0.03 uM and Kd[linear] = 3.0 ± 0.2 uM).
AssayType Ligand binding affinity

Camarero JA, Muir TW (1999) Biosynthesis of a Head-to-Tail Cyclized Protein with Improved Biological Activity. J Am Chem Soc 121:5597-8

Proteins Assayed Src homology 3 domain (Crkcirc-wt)