(17) Assay Card

General Information
Summary Circulins C-F inhibited the cytopathic effects of in vitro HIV-1 infection.
Condition The crude extract, chromatographic fractions, and purified peptides were dissolved in either 100% DMSO or H2O-DMSO (3:1), diluted to the desired concentration, and tested in an XTT-based in vitro anti-HIV assay
Result Circulins C-F inhibited the cytopathic effects of in vitro HIV-1 infection with EC50 values of 50-275 nM.
AssayType Anti-HIV

References
Gustafson KR, Walton LK, Sowder RC Jr, Johnson DG, Pannell LK, Cardellina JH Jr, Boyd MR (2000) New circulin macrocyclic polypeptides from Chassalia parvifolia. J Nat Prod 63:176-8

Cross-references
Proteins Assayed circulin C
circulin D
circulin E
circulin F