(165) Assay Card

General Information
Summary Human beta-coagulation factor XIIa (FXIIa) binding affinity (KD) of chemically synthesized MCoTI-II and acyclic or cyclic MCoFx1-5
Condition Refer "Binding Affinity Determination" described in the method part (Liu et al., 2021).
Result MCoTI-II: 58+/-8 nM; MCoFx1: 1.89+/-0.04 nM; cMCoFx1: 0.90+/-0.04 nM; MCoFx2: 45.4+/-2.9 nM; cMCoFx2: 10.0+/-1.3 nM; MCoFx3: 167+/-40 nM; cMCoFx3: 22.8+/-1.6 nM; MCoFx4: 73.8+/-6.3 nM; cMCoFx4: 14.4+/-1.1 nM; MCoFx5: 23.0+/-0.4 nM; cMCoFx5: 81.8+/-3.2 nM
AssayType Enzyme binding affinity

References
Liu W, de Veer SJ, Huang YH, Sengoku T, Okada C, Ogata K, Zdenek CN, Fry BG, Swedberg JE, Passioura T, Craik DJ, Suga H. (2021) An Ultrapotent and Selective Cyclic Peptide Inhibitor of Human beta-Factor XIIa in a Cyclotide Scaffold J Am Chem Soc 143:18481-18489

Cross-references
Proteins Assayed MCoFx1
cMCoFx1
MCoFx2
cMCoFx2
MCoFx3
cMCoFx3
MCoFx4
cMCoFx4
MCoFx5
cMCoFx5