(142) Assay Card

General Information
Summary Cycloviolacin O2 showed antibacterial activity against A. baumannii.
Condition Concentration determination of cyO2 against E. coli and A. baumannii was performed in Mueller−Hinton media, using a broth microdilution method. Bacteria were grown overnight and added to the assay at 105 CFU/mL and turbidity/clearing was observed after 20 h incubation. Assay was performed in triplicate starting with 10 or 25 μM cyO2 for E. coli or A. baumannii, respectively, and using 2× serial dilution. Mean MICs of 5 and 15 μM were obtained for each species, respectively. The mean IC50 value of 6.6 μg/mL for cyO2 against the PC3 prostate cancer cell line was determined using duplicate measurements of the MTT cell viability assay, as described above.
Result The MIC for the ESKAPE pathogen representing novel activity, A. baumannii, was determined to be 15 μM, using isolated cyO2
AssayType Anti-bacterial

Kirkpatrick, C.L., Broberg, C.A., McCool, E.N., Lee, W.J., Chao, A., McConnell, E.W., Pritchard, D.A., Hebert, M., Fleeman, R., Adams, J. and Jamil, A. (2017) The "
Pipeline for Natural Product Bioactive Peptide Discovery Analytical chemistry 89:1194-1201

Proteins Assayed cycloviolacin O2