(112) Assay Card

General Information
Summary Kalata B1 binds to Mn2+ in the presence of DPC micelles
Condition Saturating concentration of DPC. Monitored by NMR.
Result Specific attenuation of amide signals, implicating loops 1,2 and 3 to be involved in the metal binding site
AssayType Metal binding assay

References
Shenkarev ZO, Nadezhdin KD, Sobol VA, Sobol AG, Skjeldal L, Arseniev AS. (2006) Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle. FEBS J 273:2658-2672

Cross-references
Proteins Assayed kalata B1