Cyclisation Folding Free Energy Change

This tool calculates the change in folding free energy from backbone cyclisation. It uses the equations specified by Huan-Xiang Zhou in J. Mol. Biol. (2003) 332:257-264. The authors of this website are sincerely grateful for the help provided by Huan-Xiang Zhou.

Protein Details
PDB File OR Number of Residues in Protein N- to C- Termini Distance (Å)
Linker Details
Starting Linker Length (Mininum of 2, Energies for the next 5 linker lengths will also be calculated)