RTD-1(85) Protein Card

General Information
Name RTD-1
Sequence RCICTRGFCRCLCRRGVC
Class Primate
Average Mass 2081.56
Monoisotopic Mass 2079.91
m/z M+H 2080.92
ProteinType Wild type
Parent
Organism Macaca mulatta (rhesus monkey)
Notes Theta-defensin.
Cyclic Yes

Assay
Anti-bacterial
RTD-1 found to possess anti-microbial activity greater than the acyclic analogue. [...]Tang YQ et al. (1999) Science 286:498-502
RTD-1-3 shown to possess anti-bacterial activity [...]Tran D et al. (2002) J Biol Chem 277:3079-84
Membrane Binding Assay
RTD-1-3 shown to bind with equal affinity to E.coli. [...]Tran D et al. (2002) J Biol Chem 277:3079-84
RTD-1 shows two physicacally distinct states when bound to lipid bilayers [...]Weiss TM et al. (2002) Biochemistry 41:10070-6
The cyclic antimicrobial peptide RTD-1 induces stabilized lipid-peptide domains more efficiently than its open-chain analogue [...]Abuja PM et al. (2004) FEBS Lett 566:301-6
NMR used to investigate RTD-1 interaction with lipid bilayer [...]Buffy JJ et al. (2004) Biochemistry 43:9800-12

References
Tang YQ, Yuan J, Osapay G, Osapay K, Tran D, Miller CJ, Ouellette AJ, Selsted ME (1999) A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated alpha-defensins. Science 286:498-502
Trabi M, Schirra HJ, Craik DJ (2001) Three-dimensional structure of RTD-1, a cyclic antimicrobial defensin from Rhesus macaque leukocytes. Biochemistry 40:4211-21
Tran D, Tran PA, Tang YQ, Yuan J, Cole T, Selsted ME (2002) Homodimeric theta-defensins from rhesus macaque leukocytes: isolation, synthesis, antimicrobial activities, and bacterial binding properties of the cyclic peptides. J Biol Chem 277:3079-84
Weiss TM, Yang L, Ding L, Waring AJ, Lehrer RI, Huang HW (2002) Two states of cyclic antimicrobial peptide RTD-1 in lipid bilayers. Biochemistry 41:10070-6
Abuja PM, Zenz A, Trabi M, Craik DJ, Lohner K (2004) The cyclic antimicrobial peptide RTD-1 induces stabilized lipid-peptide domains more efficiently than its open-chain analogue. FEBS Lett 566:301-6
Buffy JJ, McCormick MJ, Wi S, Waring A, Lehrer RI, Hong M (2004) Solid-state NMR investigation of the selective perturbation of lipid bilayers by the cyclic antimicrobial peptide RTD-1. Biochemistry 43:9800-12

Cross-references
Nucleic acids theta defensin 1a precursor
theta defensin 1b precursor
Structure RTD-1
Links GenBank C59089

Tools