N-SFTI-1-C(82) Protein Card

General Information
Name N-SFTI-1-C
Class BBI-like trypsin inhibitor
Average Mass 1531.81
Monoisotopic Mass 1530.74
m/z M+H 1531.75
ProteinType Acyclic permutant
Parent SFTI-1
Organism Synthetic
Notes Acyclic permutant
Cyclic No

Protease inhibition
Both SFTI-1 and acyclic SFTI-1 are potent inhibitors of bovine beta trypsin [...]Korsinczky ML et al. (2001) J Mol Biol 311:579-91
SFTI-1, an acyclic permutant and a [CYS-Abu]-cyclic mutant shown to possess potent trypsin inhibitory activity. [...]Zablotna E et al. (2002) Biochem Biophys Res Commun 292:855-9
Chymotrypsin and trypsin Ka values determined for SFTI-1 analogues. [...]Stawikowski M et al. (2005) Chembiochem 6:1057-61
SFTI-1 and analogues shown to inhibit Bovine beta-trypsin and Human leukocyte elastase [...]Zablotna E et al. (2006) Biochem Biophys Res Commun 340:823-8
Disulfide bond is essenial for structure and function of acyclic SFTI-1. Seleno aSFTI-1 has 30% reduced inhibitory constant. [...]Guo X et al. (2006) Chem Biol Drug Des 68:341-4
Enzymatic Digest
Susceptibility of SFTI-1 analogues to cleavage by chymotrypsin and tryspin. [...]Stawikowski M et al. (2005) Chembiochem 6:1057-61

Korsinczky ML, Schirra HJ, Rosengren KJ, West J, Condie BA, Otvos L, Anderson MA, Craik DJ (2001) Solution structures by 1H NMR of the novel cyclic trypsin inhibitor SFTI-1 from sunflower seeds and an acyclic permutant. J Mol Biol 311:579-91
Stawikowski M, Stawikowska R, Jaskiewicz A, Zablotna E, Rolka K (2005) Examples of Peptide-Peptoid Hybrid Serine Protease Inhibitors Based on the Trypsin Inhibitor SFTI-1 with Complete Protease Resistance at the P1-P1' Reactive Site. Chembiochem 6:1057-61
Hilpert K, Hansen G, Wessner H, Volkmer-Engert R, Hohne W (2005) Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases. J Biochem (Tokyo) 138:383-90
Zablotna E, Kret A, Jaskiewicz A, Olma A, Leplawy MT, Rolka K (2006) Introduction of alpha-hydroxymethyamino acid residues in substrate specificity P1 position of trypsin inhibitor SFTI-1 from sunflower seeds retains its activity. Biochem Biophys Res Commun 340:823-8
Guo X, Shi J, Tang Z, Cui D, Zhang Y (2006) Synthesis and biological activity of seleno sunflower trypsin inhibitor analog. Chem Biol Drug Des 68:341-4

Nucleic acids
Structure N-SFTI-1-C