[V25A]kalata B1(473) Protein Card

General Information
Name [V25A]kalata B1
Sequence GLPVCGETCVGGTCNTPGCTCSWPACTRN
Class Cyclotide
Average Mass 2864.23
Monoisotopic Mass 2862.11
m/z M+H 2863.12
ProteinType Mutant
Parent kalata B1
Organism Synthetic
Notes
Cyclic Yes

Assay
Insecticidal
Substitution of certain residues of kalata B1 to alanine result in loss of insecticidal activity [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Hemolytic
Substitution of certain residues of kalata B1 to alanine result in loss of hemolytic activity. Residues in loops 1 and 3 along with a few other isolated residues strongly affect activity. [...]Simonsen SM et al. (2008) J Biol Chem 283:9805-9813
Nematocidal
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589

References
Simonsen SM, Sando L, Rosengren KJ, Wang CK, Colgrave ML, Daly NL, Craik DJ (2008) Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity J Biol Chem 283:9805-9813

Cross-references
Nucleic acids
Structure
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