kalata B2(4) Protein Card

General Information
Name kalata B2
Sequence GLPVCGETCFGGTCNTPGCSCTWPICTRD
Class Cyclotide
Technique EST,MS,Amino acid analysis
Average Mass 2955.33
Monoisotopic Mass 2953.14
m/z M+H 2955.33
ProteinType Wild type
Parent
Organism Oldenlandia affinis
Notes
Cyclic Yes

Assay
Nematocidal
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
kB1 and kB2 inhibited the hatching of H. contortus and T. colubriformis eggs in a concentration-dependent manner. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
Molluscicidal
Cyclotides show molluscicidal activity [...]Plan MR et al. (2008) J Agric Food Chem 56:5237-5241
Membrane Binding Assay
Kalata B1 and B2 cause leakage of dye-containing POPC vesicles. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
Insecticidal
H. armigera larvae fed kalata B1 or kalata B2 exhibited reduced growth and higher mortality rate compared to cyclotide-less diet [...]Jennings CV et al. (2005) Biochemistry 44:851-60
NOE Restraint
NOE restraints for 1PT4 [...]
Chemical Shift
Chemical shift data for kalata B2 [...]
Protease inhibition
Kalata B1 and B2 were found to have no trypsin or chymotrypsin inhibitory activity. [...]Jennings C et al. (2001) Proc Natl Acad Sci U S A 98:10614-9

References
Jennings CV, Rosengren KJ, Daly NL, Plan M, Stevens J, Scanlon MJ, Waine C, Norman DG, Anderson MA, Craik DJ (2005) Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist: do Mobius strips exist in nature? Biochemistry 44:851-60
Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ (2004) Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. J Biol Chem 279:46858-67
Trabi M, Craik DJ (2004) Tissue-specific expression of head-to-tail cyclized miniproteins in Violaceae and structure determination of the root cyclotide Viola hederacea root cyclotide1. Plant Cell 16:2204-16
Jennings C, West J, Waine C, Craik D, Anderson M (2001) Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis. Proc Natl Acad Sci U S A 98:10614-9
Craik DJ, Daly NL, Bond T, Waine C (1999) Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif. J Mol Biol 294:1327-36
Nourse A, Trabi M, Daly NL, Craik DJ (2004) A comparison of the self-association behavior of the plant cyclotides kalata B1 and kalata B2 via analytical ultracentrifugation. J Biol Chem 279:562-70
Plan MR, Goransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ (2007) The Cyclotide Fingerprint in Oldenlandia affinis: Elucidation of Chemically Modified, Linear and Novel Macrocyclic Peptides Chembiochem 8:1001-1011
Qin Q, McCallum EJ, Kaas Q, Suda J, Saska I, Craik DJ, Mylne JS. (2010) Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11:111-0
Burman,R., Strömstedt,A.A., Malmsten,M. and Göransson,U. (2011) Cyclotide-membrane interactions: defining factors of membrane binding, depletion and disruption. Biochim. Biophys. Acta 1808:2665-2673
Ovesen,R.G., Nielsen,J. and Hansen,H.C. (2011) Biomedicine in the environment: sorption of the cyclotide kalata B2 to montmorillonite, goethite, and humic acid. Environ. Toxicol. Chem. 30:1785-1792

Cross-references
Protein precursor(s) kalata B2 precursor
kalata B2 precursor
kalata B2 precursor
Kalata B2 precursor
Kalata B2 precursor
Kalata B2 precursor
Nucleic acids kalata B2 precursor [OaK4]
Structure Solution structure of the Moebius cyclotide kalata B2
Solution structure of micelle-bound kalata B2
Links GenBank P58454.1
SwissProt P58454

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