kalata B7(26) Protein Card

General Information
Name kalata B7
Sequence GLPVCGETCTLGTCYTQGCTCSWPICKRN
Class Cyclotide
Technique EST,Amino acid analysis,MS
Average Mass 3071.54
Monoisotopic Mass 3069.27
m/z M+H 3071.54
ProteinType Wild type
Parent
Organism Oldenlandia affinis
Notes
Cyclic Yes

Assay
Nematocidal
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
Molluscicidal
Cyclotides show molluscicidal activity [...]Plan MR et al. (2008) J Agric Food Chem 56:5237-5241
Metal binding assay
Kalata B7 binds to Mn2+ in the presence of DPC micelles [...]Shenkarev ZO et al. (2008) J Inorg Biochem 102:1246-1256
Membrane Binding Assay
Kalata B7 binds to DPC micelles [...]Shenkarev ZO et al. (2008) J Inorg Biochem 102:1246-1256

References
Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ (2004) Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. J Biol Chem 279:46858-67
Jennings C, West J, Waine C, Craik D, Anderson M (2001) Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis. Proc Natl Acad Sci U S A 98:10614-9
Plan MR, Goransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ (2007) The Cyclotide Fingerprint in Oldenlandia affinis: Elucidation of Chemically Modified, Linear and Novel Macrocyclic Peptides Chembiochem 8:1001-1011
Qin Q, McCallum EJ, Kaas Q, Suda J, Saska I, Craik DJ, Mylne JS. (2010) Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11:111-0

Cross-references
Protein precursor(s) kalata B7 precursor
Nucleic acids kalata B7 precursor [OaK3]
Structure NMR spatial srtucture of ternary complex kalata B7/Mn2+/DPC micelle
Links GenBank AAL05478.1
SwissProt P58457

Tools