kalata B6(24) Protein Card

General Information
Name kalata B6
Sequence GLPTCGETCFGGTCNTPGCSCSSWPICTRN
Class Cyclotide
Technique EST,Amino acid analysis,MS
Average Mass 3029.37
Monoisotopic Mass 3027.15
m/z M+H 3029.37
ProteinType Wild type
Parent
Organism Oldenlandia affinis
Notes
Cyclic Yes

Assay
Nematocidal
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945
The cyclotides caused a concentration-dependent response in the LDA with the two nematode species. [...]Colgrave ML et al. (2008) Biochemistry 47:5581-5589
Membrane Binding Assay
Showed that kalata B1 and B6 would bind selectively to bacterial membranes. [...]Kamimori H et al. (2005) Anal Biochem 337:149-53

References
Dutton JL, Renda RF, Waine C, Clark RJ, Daly NL, Jennings CV, Anderson MA, Craik DJ (2004) Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. J Biol Chem 279:46858-67
Jennings C, West J, Waine C, Craik D, Anderson M (2001) Biosynthesis and insecticidal properties of plant cyclotides: the cyclic knotted proteins from Oldenlandia affinis. Proc Natl Acad Sci U S A 98:10614-9
Kamimori H, Hall K, Craik DJ, Aguilar MI (2005) Studies on the membrane interactions of the cyclotides kalata B1 and kalata B6 on model membrane systems by surface plasmon resonance. Anal Biochem 337:149-53
Plan MR, Goransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ (2007) The Cyclotide Fingerprint in Oldenlandia affinis: Elucidation of Chemically Modified, Linear and Novel Macrocyclic Peptides Chembiochem 8:1001-1011
Qin Q, McCallum EJ, Kaas Q, Suda J, Saska I, Craik DJ, Mylne JS. (2010) Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11:111-0

Cross-references
Protein precursor(s) kalata B3/B6 precursor
Nucleic acids kalata B3/B6 precursor [OaK2]
Structure
Links GenBank AAL05478.1
SwissProt P58455-b3

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