cycloviolacin O16(176) Protein Card

General Information
Name cycloviolacin O16
Sequence GLPCGETCFTGKCYTPGCSCSYPICKKIN
Class Cyclotide
Average Mass 3050.56
Monoisotopic Mass 3048.28
m/z M+H 3049.28
ProteinType Wild type
Parent
Organism Viola odorata
Notes
Cyclic Yes

Assay
Enzymatic Digest
Cycloviolacin O13, O14, O16 and O24 were tested for their stability against proteolytic degradation by pepsin, trypsin and thermolysin.No degradation by proteases was observed for any of the cyclotides tested following 6 hours of incubation with enzymes, while the linear control peptide was completely degraded in less than 5 minutes. [...]Ireland DC et al. (2006) Biochem J. 400:1-12
Nematocidal
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945

References
Ireland DC, Colgrave ML, Craik DJ (2006) A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability. Biochem J. 400:1-12

Cross-references
Nucleic acids
Structure
Links SwissProt P85179

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