(82) Assay Card

General Information
Summary Glutamic acid mutant had reduced activity
Condition Human lymphoma cell line U-937 GTB used in fluorometric microculture cytotoxicity assay
Result IC50 (uM): cyO2 (0.75), [Glu(Me)]cyO2 (36), [Lys(Ac)]2cyO2 (2.3), [Arg(CHD)]cyO2 (0.95), ([Lys(Ac)]2[Arg(CHD)])cyO2 5.1, [Cys(CAM)]6cyO2 (> 80)
AssayType Cancer Cell Toxicity

References
Herrmann A, Svangard E, Claeson P, Gullbo J, Bohlin L, Goransson U (2006) Key role of glutamic acid for the cytotoxic activity of the cyclotide cycloviolacin O2. Cell Mol Life Sci 63:235-45

Cross-references
Proteins Assayed cycloviolacin O2
[Glu(Me)]cyO2
[Lys(Ac)]2cyO2
[Arg(CHD)]cyO2
([Lys(Ac)]2[Arg(CHD)])cyO2