(76) Assay Card

General Information
Summary Kalata B8 showed anti-HIV and cytotoxic activity. Tricyclon A did not show anti-HIV or cytotoxic activity.
Condition In vitro XTT based anti-HIV assay.
Result Anti-HIV tested for kalata B8 (4.5 mM) and tricyclon A (5.8 mM). Kalata B8 inhibited cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells with antiviral cytoprotective concentration (EC50) ~ 2.5 mM, with cytotoxic concentration (IC50) > 11 mM. Tricyclon A did not have anti-HIV ro cytotoxic activity.Anti-HIV tested for kalata B8 (4.5 mM) and tricyclon A (5.8 mM). Kalata B8 inhibited cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells with antiviral cytoprotective concentration (EC50) ~ 2.5 mM, with cytotoxic concentration (IC50) > 11 mM. Tricyclon A did not have anti-HIV ro cytotoxic activity.
AssayType Anti-HIV

References
Daly NL, Clark RJ, Plan MR, Craik DJ (2006) Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif. Biochem J 393:619-26

Cross-references
Proteins Assayed tricyclon A
kalata B8