(73) Assay Card

General Information
Summary Open chain variants have reduced inhibitory activity against bovine trypsin compared to native cyclic proteins.
Condition Trypsin incubated with dilutions of peptide in 50 mM Trish-HCl, 150 mM NaCl, 0.01% Triton X-100, 0.01% sodium azide, pH 7.6.
Result Ki (nM): oMCoTI-II (0.3), [A10]oMCoTI-II (nd), [A13]oMCoTI-II (0.2), [A14]oMCoTI-II (0.4), [R10]oMCoTI-II (0.8), [R13]oMCoTI-II (0.5), [R14]oMCoTI-II (0.4), [AlaG10]oMCoTI-II (1000), MCoTI-I (0.02), MCoTI-II (0.03), MCoTI-III (0.5)
AssayType Protease inhibition

References
Avrutina O, Schmoldt H, Gabrijelcic-Geiger D, Le Nguyen D, Sommerhoff CP, Diederichsen U, Kolmar H (2005) Trypsin inhibition by macrocyclic and open-chain variants of the squash inhibitor MCoTI-II. Biol Chem 386:1301-6

Cross-references
Proteins Assayed MCoTI-II
oMCoTI-II
[A10]oMCoTI-II
[A13]oMCoTI-II
[A14]oMCoTI-II
[R10]oMCoTI-II
[R13]oMCoTI-II
[R14]oMCoTI-II
[AlaG10]oMCoTI-II
MCoTI-I