(69) Assay Card

General Information
Summary 12 SFTI alanine mutants were tested for inhibitory activity against bovine trypsin. K5A mutant shows the weakest activity.
Condition Trypsin incubated with dilutions of peptides in 50 mM HEPES, 150 MM NaCl, 0.01% Triton X-100, 0.01% sodium azide, pH 7.4, 5 min.
Result Ki (nM): SFTI-1 (0.03), G1A (0.08), R2A (0.47), T4A (0.26), K5A (190), S6A (0.35), I7A (0.84), P8A (1.49), P9A (0.08), I10A (0.11), F12A (0.53), P13A (0.05), D14A (0.24)
AssayType Protease inhibition

Daly NL, Chen YK, Foley FM, Bansal PS, Bharathi R, Clark RJ, Sommerhoff CP, Craik DJ (2006) The absolute structural requirement for a proline in the P3'-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold J Biol Chem 281:23668-75

Proteins Assayed SFTI-1