(66) Assay Card

General Information
Summary SFTI based variants shown to have activity against Porcine pancreatic elastase and Proteinase K
Condition Hydrolysis monitored at 400 nm by titrating at 25 degrees Celsius, in 0.1 M Tris pH 8.5 for PPE and pH 8.0 for proteinase K.
Result Ki values for PPE and Proteinase K (M): OSFEI I (3.5E-5, nd), OSFEI II (3.0E-7, 1.1E-4), OSFPKI I (nd, 3.9E-4), OSFPKI II (nd, 2.1E-4), OSFPKI III (4.8E-5, 7.4E-6), HEI-TOS (7.3E-6, nd)
AssayType Protease inhibition

References
Hilpert K, Hansen G, Wessner H, Volkmer-Engert R, Hohne W (2005) Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases. J Biochem (Tokyo) 138:383-90

Cross-references
Proteins Assayed OSFEI I
OSFEI II
OSFPKI I
OSFPKI II
OSFPKI III
HEI-TOS