(134) Assay Card

General Information
Summary HB5-9 showed significant cytotoxicity on cancer cell lines BxPC3, Capan2, MOH1 and PANC1. HB7 inhibited the cell migration and invasion of Capan2 cells and could significantly inhibit the tumour weight and size.
Condition The cytotoxicity of cyclotides HB5 to HB9 were assessed against pancreatic cancer cells. Briefly, cells were seeded in 96-well flat-bottomed microtiter plates (2x10^3).After culturing for 12 h, cells were treated with cyclotides at 0.1, 0.5, 1, 4, 10 and 20 μM in 200 μL of media for 72 h. The medium was removed from each well and 100 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT; 0.5 mg/mL in PBS) was added in the absence of light; formazan crystals were produced over a 4h incubation period. To dissolve crystals, 150 μL of 0.04 N HCl in isopropanol was added to each well, and the optical density at 570 nm was measured on a spectrophotometer (MD, USA).
Result IC50 of HB5 against cancer cell lines BxPC3, Capan2, MOH1 and PANC1 was 1.03 uM, 1.21uM, 1.32uM, 1.08uM. IC50 of HB6 against cancer cell lines BxPC3, Capan2, MOH1 and PANC1 was 2.33 uM, 1.85 uM, 2.10 uM, 1.97 uM. IC50 of HB7 against cancer cell lines BxPC3, Capan2, MOH1 and PANC1 was 0.68 uM, 0.45 uM, 0.33 uM, 0.36 uM. IC50 of HB8 against cancer cell lines BxPC3, Capan2, MOH1 and PANC1 was 3.11 uM, 2.14 uM, 1.88 uM, 1.95 uM. IC50 of HB9 against cancer cell lines BxPC3, Capan2, MOH1 and PANC1 was 1.54 uM, 1.32 uM, 1.14 uM, 2.01 uM.
AssayType Cancer Cell Toxicity

References
Ding, X., Bai, D. and Qian, J (2014) Novel cyclotides from Hedyotis biflora inhibit proliferation and migration of pancreatic cancer cell in vitro and in vivo Medicinal Chemistry Research 23:1406-1413

Cross-references
Proteins Assayed HB9
HB8
HB7
HB6
HB5